Advisory Committee Chair
Bin Ren
Advisory Committee Members
Yabing Chen
Adam Beck
Bart Rose
James George
Document Type
Dissertation
Date of Award
1-1-2025
Degree Name by School
Doctor of Philosophy (PhD) Heersink School of Medicine
Abstract
Vascular endothelial cells (ECs) are increasingly recognized as a distinct class of innate immune cells, with CD36, an innate immunity receptor, playing a pivotal role in regulating immune function and its regulation, angiogenesis, cancer progression, brain related disorder as well as the exchange of nutrients and waste. To investigate this further, we developed EC-specific CD36 knockout mice using the translating ribosome affinity purification (TRAP) technique. By isolating ribosome-bound mRNA directly from vascular endothelial cells in vivo in these knockout and control mice, we conducted deep RNA sequencing and transcriptional profiling. Our findings revealed that CD36 deficiency in brain endothelia led to the downregulation of gene signatures associated with innate immune memory, alongside changes in the expression of genes related to innate immunity and immune responses, which were linked to fatty acid (FA) metabolism. Additionally, single-nuclear RNA-seq analysis of brain tissues from Alzheimer’s disease (AD) patients highlighted the critical involvement of a subset of CD36-positive brain ECs in disease progression. . Endothelial cells in blood vessels and lymph nodes play a crucial role in regulating the spread of breast cancer. They contribute to tumor growth and metastasis by secreting various factors that activate breast cancer cells, including cytokines and breast cancer stem cell-related signals. To further elucidate the significance of endothelial cells in breast cancer progression, we examined the viability, migration, proliferation, and apoptotic potential of ER+ breast cancer cells. Our findings demonstrate that the combination of Fc3TSR and Everolimus effectively inhibits the growth of ER-positive breast cancer cells.
Recommended Citation
Kumar, Atul, "Endothelial Cell Cd36 In Alzheimer’S Disease And Breast Cancer Progression" (2025). All ETDs from UAB. 6860.
https://digitalcommons.library.uab.edu/etd-collection/6860
Comments
etdadmin_upload_1138018