Advisory Committee Chair
Sadis Matalon
Advisory Committee Members
Michelle V Fanucchi
Rakesh P Patel
Edward M Postlethwait
Document Type
Thesis
Date of Award
2010
Degree Name by School
Master of Science in Public Health (MSPH) School of Public Health
Abstract
Rationale: Nitrite (NO2-) has been shown to limit injury to the heart, liver and kidneys in various models of ischemia-reperfusion injury. Currently, potential protective effects of systemic NO2- in limiting lung injury or enhancing repair have not been documented. Objectives: To assess the efficacy and mechanisms by which post-exposure intra-peritoneal injections of NO2- mitigate Cl2 induced lung injury in rats. Methods: Rats were exposed to Cl2 (400 ppm) for 30 minutes and returned to room air. Nitrite (1mg/Kg) or saline were administered intraperitoneally at 10 min, 2, 4 and 6 hrs post exposure. Rats were sacrificed at 6 hrs or 24 hrs and injury to airway and alveolar epithelia was assessed by morphology, protein concentrations, number of cells in bronchoalveolar lavage (BAL) and wet/dry lung weights. Lipid peroxidation was assessed by measuring lung F2-isoprostanes. Results: Rats developed severe but transient hypoxemia. At 6 hrs post-Cl2 exposure, protein concentration, the number of neutrophils, and lung wet/dry weights increased significantly. Quantitative morphology revealed extensive lung injury in the upper airways; airway epithelial cells stained positive for TUNEL, but not caspase-3. Administration of NO2- resulted in lower BAL protein levels, significant reduction in the intensity of the TUNEL positive cells and normal values of lung wet/dry weights. F2-isoprostane levels increased at six and 24 h post Cl2 exposure in both NO2- and saline injected rats. Conclusion: This is the first time demonstration that systemic nitrite administration mitigates airway and epithelial injury. Key words: pulmonary edema, apoptosis, necrosis, isoprostanes, airway injury
Recommended Citation
Yadav, Amit Kumar, "Therapeutic Interventions for Chlorine gas induced Lung Injury" (2010). All ETDs from UAB. 3394.
https://digitalcommons.library.uab.edu/etd-collection/3394