Advisory Committee Chair
Ching-Yi Chen
Advisory Committee Members
David M Bedwell
Louise T Chow
Hengbin Wang
Martin E Young
Document Type
Dissertation
Date of Award
2014
Degree Name by School
Doctor of Philosophy (PhD) Heersink School of Medicine
Abstract
White adipose tissue (WAT) releases fatty acids from stored triacylglycerol (TAG) for energy source. Here, we report that null mice for KH-type splicing regulatory protein (KSRP), an RNA-binding protein, resisted high fat diet (HFD) induced obesity. Target of deletion of KSRP regulates gene expression at multiple levels and enhances lipolysis in epididymal WAT (eWAT) due to up-regulation of genes promoting lipolytic activity. Expression of miR-145 is decreased due to impaired pri-miR-145 processing in Ksrp-/- eWAT. We show that miR-145 directly targets and represses Foxo1 and Cgi58, activators of lipolytic activity, and forced expression of miR-145 attenuates lipolysis. This study reveals a novel in vivo function of KSRP in controlling adipose lipolysis through post-transcriptional regulation of miR-145 expression.
Recommended Citation
Lin, Yi-Yu, "Ksrp Ablation Enhances Lipolysis In White Adipose Tissue Through Reduced Mir-145 Expression" (2014). All ETDs from UAB. 2295.
https://digitalcommons.library.uab.edu/etd-collection/2295