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Advisory Committee Chair

Paul D Eleazer

Advisory Committee Members

Noel K Childers

Amjad Javed

Document Type

Thesis

Date of Award

2018

Degree Name by School

Master of Science in Dentistry (MScD) School of Dentistry

Abstract

Purpose: To investigate the effects of three novel experimental antibiotics on monospecies (Enterococcus faecalis) and polymicrobial (mixed) endodontic biofilms. Materials and Methods: Biofilms containing pure Enterococcus faecalis, as well as biofilms derived from mixed bacterial samples aspirated from inside the pulp spaces of necrotic human teeth, were grown onto slices of human dental in vitro. Biofilm formation capability was verified with SEM. The treatment groups consisted of 3 novel experimental antibiotics. 8.25% sodium hypochlorite and sterile saline were used as the controls. The biofilms were investigated with live/dead fluorochrome staining and confocal laser scanning microscopy. Results: The full strength sodium hypochlorite was capable of removing the biofilms in their entirety. The novel experimental antibiotics were shown to significantly remove bacteria under different exposure parameters, however they could not completely kill all microbes within these biofilms. Conclusion: The novel experimental antibiotics were not capable of completely removing monospecies (Enterococcus faecalis) or polymicrobial (mixed) endodontic biofilms grown on slices of human dentin.

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