All ETDs from UAB

Advisory Committee Chair

Stephen A Watts

Advisory Committee Members

Addison L Lawrence

Louis D’Abramo

James B McClintock

Mickie L Powell

Document Type


Date of Award


Degree Name by School

Doctor of Philosophy (PhD) College of Arts and Sciences


The globally growing human population has increased the needs for protein based food. Single cell proteins (SCPs) as unconventional protein source, when coupled to aq-uaculture production, offer a means to ensure future protein needs can be met. To demon-strate a given type of SCP has potential as a protein source for use in aquaculture feed; a number of investigations are needed including demonstrating that the SCP is attractable and accepted by the animals, leads to equivalent survival, growth and nutritional compo-sition. This dissertation represents a series of investigations designed to evaluate the fea-sibility of using a bacterial based (MRD-Pro) and yeast based (DY-Pro) SCPs as alterna-tive protein ingredients to substitute FM protein in pacific white shrimp Litopenaeus van-namei. In chapter 2, a bioassay which quantifies feed intake as an estimation of relative attractability of feeds containing different ingredients in L.vannamei feed, is described. Chapter 3 investigates and compares the relative attractability of these two SCP products in L. vannamei feeds by using the bioassay and statistic method developed in chapter 2. In chapter 4, the effects of MRD-Pro and DY-Pro SCPs replacing 32% and 76% of FM protein in diets respectively with identical nutrient levels on growth performance and body composition of L. vannamei are explored. In chapter 5, a feeding trial is conducted to investigate the effects of replacement of FM protein with different proportions of MRD-Pro and DY-Pro SCPs on growth performance and body composition of L. van-namei. The attractability assay results indicated that diets containing 6.9% DY-Pro, 6.9% MRD-Pro or 16.3% MRD-Pro had significantly greater probability of being consumed than the FM control diet. These data indicate that SCPs enhance feed intake compared to FM and are effective attractants in shrimp diets. The growth trails results indicated that growth performance and body composition values of shrimp fed experimental diets were either significantly improved or were not significantly influenced by the replacement of fish meal with DY-Pro or MRD-Pro SCPs or different proportions of DY-Pro and MRD-Pro SCPs. These data suggested that MRD-Pro, DY-Pro SCPs or a mixture of MRD-Pro and DY-Pro can be considered as potential protein ingredients to partially or fully substi-tute FM protein in commercial shrimp feed.



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