All ETDs from UAB

Advisory Committee Chair

Yogesh L Vohra

Advisory Committee Members

Susan L Bellis

Derrick R Dean

Alan W Eberhardt

Vinoy Thomas

Document Type


Date of Award


Degree Name by School

Doctor of Philosophy (PhD) School of Engineering


A bi-layer electrospun nano-fibrous scaffold comprising synthetic polymers and natural proteins was constructed as the prototype of an arterial substitute for coronary ar-tery disease. This scaffold featured a highly porous microstructure analogous to native extracellular matrix. Uni-axial tensile test suggested that its excellent mechanical strength could restore normal blood flow immediately after implantation. Subsequent biocompati-bility study demonstrated that scaffolding materials, either traditional or degraded, were non-toxic to the regenerated human endothelium on the luminal layer. Moreover, this re-generated endothelium possessed critical functions, such as the formation of tight junc-tions, the secretion of prostacyclin, and the prevention of platelet adhesion. Thereafter, porcine endothelial progenitor cells (EPCs) harvested from the peripheral blood were used to explore the in situ regeneration of a functional endothelium. The EPCs constructed within 24 hours a confluent endothelial layer that was capable of preventing platelet adhesion, suggesting that this scaffold would provide a favorable milieu in vivo for the arterial regeneration.

Included in

Engineering Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.