Advisory Committee Chair
Advisory Committee Members
Date of Award
Degree Name by School
Master of Science (MS) School of Dentistry
Purpose: Novel endodontic antimicrobial agents would potentially improve outcomes of endodontic treatment, including regenerative endodontic therapy. The aim of this study was to determine the efficacy of a novel experimental compound, analogue #66, compared to tigecycline and other controls in an ex-vivo model. Materials and Methods: MIC determination with tigecycline and analogue #66 was done for Streptococcus mutans and Streptococcus intermedius. Forty single rooted extracted teeth were inoculated weekly for three weeks with S. intermedius to create a biofilm. All teeth were instrumented to 40/06 to working length and irrigated with 5% NaOCl. Teeth were randomly divided into 4 groups (n=10 per group): (1) DMSO as negative control, (2) calcium hydroxide as positive control, (3) analogue #66 (10 mg/mL) group and (4) tigecycline (10 mg/mL). Microbiological sampling of all groups was performed after one week. Results: Log colony forming units (CFUs) from calcium hydroxide (5.07 + 0.12), analogue #66 (4.94 + 0.15) and tigecycline (0.58 + 1.22) showed statistically significant (P < 0.05) bacterial reduction when compared with negative control (5.71 + 0.17) (P<0.05). Although higher microbial reduction was observed in analogue #66 compared with calcium hydroxide, the difference was not statistically significant (P >0.05). Residual CFUs were lowest in the tigecycline group and were significantly lower than all other groups (P <0.05). Conclusion: Calcium hydroxide, analogue #66 and tigecycline all showed significant antimicrobial efficacy against S. intermedius biofilm. Tigecycline was the most efficacious in this model.
Liu, Ziqi Sally, "Relative Efficacy of Innovative Endodontic Antimicrobial Agents" (2023). All ETDs from UAB. 408.