Advisory Committee Chair
Nathaniel C Lawson
Advisory Committee Members
Date of Award
Degree Name by School
Master of Science (MS) School of Dentistry
Purpose: The purpose of this study was to assess the in-vitro antibacterial activity, biocompatibility, and mineralization-inducing potential of Biodentine® XP. Methods: Four contemporary pulp capping materials, namely Dycal®, a calcium hydroxide, Theracal® LC, a resin calcium silicate and, tricalcium silicates namely Biodentine®, and Biodentine® XP were compared in the study. The antibacterial activity of the pulp capping materials included in the study was evaluated against Streptococcus mutans UA159 and Enterococcus faecalis ATCC 29212 and were determined by the direct culture test. The biocompatibility of these materials in preodontoblastic 17IIA11 cells were compared by the MTT assay and mineralization-inducing potential in preodontoblastic 17IIA11 cells were compared by quantifying calcium deposits on Alizarin Red Staining (ARS) on day 5. Data was expressed as mean ± SEM (standard error of mean) and was evaluated statistically by one-way ANOVA followed by Tukey’s multiple comparison test using GraphPad Prism 9 (GraphPad Software Inc, San Diego, California). Differences between groups was considered significant at p<0.05. Results: In direct culture test, Biodentine® XP showed strongest antibacterial activity against S. mutans whereas Biodentine® and Biodentine® XP showed highest antibacterial activity against E. faecalis. However, all the tested pulp capping materials showed distinct antibacterial effects against S. mutans and iv E. faecalis. Of the tested materials, acceptable biocompatibility was shown by Biodentine® XP and Biodentine®. Biodentine® XP, Biodentine® and Theracal® LC have shown favorable mineralization-inducing potential in our experiment. Of all the tested materials, Dycal showed the least antibacterial activity, biocompatibility, and mineralizationinducing potential. Conclusion: Biodentine® XP showed acceptable antibacterial activity, biocompatibility, and mineralization-inducing potential of all the tested materials. Therefore, the new material can be used as a pulp capping material owing to its wide array of clinical implications and ease of use. However, more research is necessary.
Mantri, Chandani Arjun, "An In Vitro Assessment of the Antibacterial Activity, Biocompatibility, and Mineralization Inducing Potential of Biodentine®XP" (2023). All ETDs from UAB. 409.