All ETDs from UAB

Advisory Committee Chair

Ping Zhang

Advisory Committee Members

Amjad Javed

Janice Jackson

Nathaniel Lawson

Document Type


Date of Award


Degree Name by School

Master of Science (MS) School of Dentistry


Purpose: The purpose of this study was to evaluate the antibacterial activity, biocompatibility, and mineralization-inducing potential of calcium silicate-based pulp capping materials. Methods: Six contemporary calcium silicate-based pulp capping materials were compared in this study, including ProRoot MTA, MTA Angelus, Biodentine, EndoSequence, NeoMTA 2 and NeoPutty. The antibacterial effects of these materials against Streptococcus mutans UA159 and Enterococcus faecalis ATCC 29212 were determined by the agar-diffusion assay and the direct culture test. The biocompatibility and mineralization-inducing potential of these materials in preodontoblastic 17IIA11 cells were compared by the MTT assay and by evaluating alkaline phosphatase activity and calcium deposits, respectively. Results: In agar diffusion test, only Biodentine showed distinct antibacterial effects against S. mutans. However, all the tested materials showed antibacterial effects against S. mutans and E. faecalis in the direct culture test, with Biodentine showing the strongest growth inhibition against both S. mutans and E. faecalis. All the tested materials show acceptable biocompatibility. Biodentine, MTA angelus, EndoSequence, and ProRoot MTA have good mineralization-inducing potential in our experimental conditions. Conclusions: Different calcium silicate-based pulp capping materials have different antibacterial activity, biocompatibility, and mineralization-inducing potential. Understanding the iv biological properties of pulp capping materials will provide a mechanistic base of their clinical performance on maintaining pulp vitality and will guide the development of new pulp capping materials.

Included in

Dentistry Commons



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