All ETDs from UAB

Advisory Committee Chair

Ho-Wook Jun

Advisory Committee Members

Jun Chen

Kyounga Cheon

Document Type


Date of Award


Degree Name by School

Master of Biomedical Engineering (MBE) School of Engineering


Percutaneous osseointegrated prostheses (POP) implants combat the multitude of issues and limitations of contemporary prosthetic sockets/limbs, including range of motion, comfort/fit, irritation, gait, and osseoproprioception. However, POP implants lack long-term clinical success; specifically, several risks exist at the bone-implant interface, including superficial/deep infections, inflammation, poor vascularization, and inadequate osseointegration, resulting in implant loosening over time and ultimately removal of the implant. The goal of this thesis is to evaluate a multifunctional, nitric oxide-releasing bionanomatrix incorporated with antibiotic-releasing liposomes on osseointegration, inflammation, vascularization/angiogenesis, and bacterial infection. We successfully synthesized the bionanomatrix, and the antibiotic-releasing liposomes readily encapsulated the antibiotics. The antibiotic-releasing liposomes demonstrated high antibacterial efficacy in two experiments, with agar diffusion showing equivalent antibacterial effect as the stock antibiotics. The bionanomatrix coating did not affect human aortic endothelial cell (hAEC) proliferation yet improved hAEC nitric oxide production in a concentration-dependent manner compared to uncoated titanium. Next, the bionanomatrix coating re-duced pro-inflammatory monocyte proliferation and macrophage adhesion in a concen-tration-dependent manner. Furthermore, the bionanomatrix coating reduced pro-inflam-matory cytokine secretion, including interleukin-1 beta, tumor necrosis factor alpha, and interleukin-6, from lipopolysaccharide (LPS)-stimulated macrophages. In these findings, we successfully demonstrated the bionanomatrix and antibiotic-releasing liposomes can improve the critical factors of POP implant longevity. Through the completion of this thesis, we expect our bionanomatrix and antibiotic-releasing liposomes can improve the clinical outcomes and success in POP, or orthopedic, implant applications and thus improve patients’ quality of life.

Included in

Engineering Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.