Advisory Committee Chair
Advisory Committee Members
Date of Award
Degree Name by School
Master of Science (MS) School of Engineering
Percutaneous osseointegrated prostheses (POP) implants combat the multitude of issues and limitations of contemporary prosthetic sockets/limbs, including range of motion, comfort/fit, irritation, gait, and osseoproprioception. However, POP implants lack long-term clinical success; specifically, several risks exist at the bone-implant interface, including superficial/deep infections, inflammation, poor vascularization, and inadequate osseointegration, resulting in implant loosening over time and ultimately removal of the implant. The goal of this thesis is to evaluate a multifunctional, nitric oxide-releasing bionanomatrix incorporated with antibiotic-releasing liposomes on osseointegration, inflammation, vascularization/angiogenesis, and bacterial infection. We successfully synthesized the bionanomatrix, and the antibiotic-releasing liposomes readily encapsulated the antibiotics. The antibiotic-releasing liposomes demonstrated high antibacterial efficacy in two experiments, with agar diffusion showing equivalent antibacterial effect as the stock antibiotics. The bionanomatrix coating did not affect human aortic endothelial cell (hAEC) proliferation yet improved hAEC nitric oxide production in a concentration-dependent manner compared to uncoated titanium. Next, the bionanomatrix coating re-duced pro-inflammatory monocyte proliferation and macrophage adhesion in a concen-tration-dependent manner. Furthermore, the bionanomatrix coating reduced pro-inflam-matory cytokine secretion, including interleukin-1 beta, tumor necrosis factor alpha, and interleukin-6, from lipopolysaccharide (LPS)-stimulated macrophages. In these findings, we successfully demonstrated the bionanomatrix and antibiotic-releasing liposomes can improve the critical factors of POP implant longevity. Through the completion of this thesis, we expect our bionanomatrix and antibiotic-releasing liposomes can improve the clinical outcomes and success in POP, or orthopedic, implant applications and thus improve patients’ quality of life.
Estep, Benjamin Kade, "Synthesis, Characterization, and Evaluation of a Bionanomatrix and Antibiotic-Releasing Liposomes on Osseointegration, Vascularization, Inflammation, and Antibacterial Efficacy" (2023). All ETDs from UAB. 72.