Advisory Committee Chair
Jack Lemons
Advisory Committee Members
John O Burgess
Patricia Devilliers
Amjad Javed
Lance C Ramp
Document Type
Thesis
Date of Award
2012
Degree Name by School
Master of Science in Dentistry (MScD) School of Dentistry
Abstract
Objective: This investigation was to evaluate (>10 year) bone response around custom (HA plasma coated) dental implants and HA particulate. Materials and Methods: Three individual post mortem mandibular COI Implants with surrounding bone were processed at six transverse locations to obtain non-decalcified ground sections (20-80µm)(n=36) and stained initially with methylene blue/basic fuchsin. Slides were evaluated to select (n= 8) slides for regions of interest along the perimeter of the implant, using optical microscopy (Olympus BX51 microscope) with a Retiga EXi color digital camera (Olympus, Center Valley, PA) and Bioquant VR software (R&M Biometrics, Nashville, TN) at an original magnification from 1-100X and examining 1 mm around implant component. Additional non-decalcified sections were processed (n=5), TRAP stained and examined, focused on osteoclast cells that were identified by TRAP staining. Histomorphometric analyses showed peri-implant area coverage of residual CaP coating at 46.6% for all specimens. TRAP staining showed the most osteoclastic activity along CaP surfaces, followed by the CaP implant-coating and the residual alloy implant to bone contacting regions. Due to a small sample size statistical analyses were not utilized. Conclusions: Analyses of bone-implant interfaces and osteoclasts from non-decalcified sections of three patients (>10 year) post mortem custom dental implants showed very different interactions where CaP implant-coatings were being replaced, particulate CaP were partially replaced, and osseous integration along all types of implant surfaces was maintained.
Recommended Citation
Kojic, Dave Darko, "Histomorphometry and Quantitative Analysis of Longer-Term Implant Interfaces" (2012). All ETDs from UAB. 2171.
https://digitalcommons.library.uab.edu/etd-collection/2171