Advisory Committee Chair
Advisory Committee Members
John O Burgess
Lance C Ramp
Date of Award
Degree Name by School
Master of Science in Dentistry (MScD) School of Dentistry
Objective: This investigation was to evaluate (>10 year) bone response around custom (HA plasma coated) dental implants and HA particulate. Materials and Methods: Three individual post mortem mandibular COI Implants with surrounding bone were processed at six transverse locations to obtain non-decalcified ground sections (20-80µm)(n=36) and stained initially with methylene blue/basic fuchsin. Slides were evaluated to select (n= 8) slides for regions of interest along the perimeter of the implant, using optical microscopy (Olympus BX51 microscope) with a Retiga EXi color digital camera (Olympus, Center Valley, PA) and Bioquant VR software (R&M Biometrics, Nashville, TN) at an original magnification from 1-100X and examining 1 mm around implant component. Additional non-decalcified sections were processed (n=5), TRAP stained and examined, focused on osteoclast cells that were identified by TRAP staining. Histomorphometric analyses showed peri-implant area coverage of residual CaP coating at 46.6% for all specimens. TRAP staining showed the most osteoclastic activity along CaP surfaces, followed by the CaP implant-coating and the residual alloy implant to bone contacting regions. Due to a small sample size statistical analyses were not utilized. Conclusions: Analyses of bone-implant interfaces and osteoclasts from non-decalcified sections of three patients (>10 year) post mortem custom dental implants showed very different interactions where CaP implant-coatings were being replaced, particulate CaP were partially replaced, and osseous integration along all types of implant surfaces was maintained.
Kojic, Dave Darko, "Histomorphometry and Quantitative Analysis of Longer-Term Implant Interfaces" (2012). All ETDs from UAB. 2171.