Advisory Committee Chair
Stephen A Watts
Advisory Committee Members
James A Bibb
Gregory D Kennedy
Document Type
Thesis
Date of Award
2019
Degree Name by School
Master of Science (MS) College of Arts and Sciences
Abstract
Inflammatory Bowel Disease (IBD) is a group of idiopathic chronic inflammatory intestinal conditions. To reveal its pathogenesis, several different animal models have been established. Although no single model captures the complexity of human IBD, each model provides valuable insights into one or another major aspect of disease, and together they have led to the establishment of a generally accepted understanding for human IBD pathogenesis. Recently, zebrafish have become an important platform for IBD research, and several murine IBD outcomes have been adapted to the zebrafish. Dextran Sulfate Sodium (DSS) -induced colitis, the most widely used murine IBD model, has never been successfully implemented in adult zebrafish. In this study we induced IBD in adult zebrafish by DSS with different methods, then compared its histological features and cytokine induction profiles with previous IBD models for validation. After successfully observing characteristics similar to murine IBD in the DSS-treated adult zebrafish, we reported the establishment of DSS-induced IBD adult zebrafish model.
Recommended Citation
Zeng, Ningxiang, "Methods for the establishment of a DSS-induced IBD model in adult zebrafish" (2019). All ETDs from UAB. 3434.
https://digitalcommons.library.uab.edu/etd-collection/3434