All ETDs from UAB

Advisory Committee Chair

Stephen A Watts

Advisory Committee Members

James A Bibb

Gregory D Kennedy

Document Type

Thesis

Date of Award

2019

Degree Name by School

Master of Science (MS) College of Arts and Sciences

Abstract

Inflammatory Bowel Disease (IBD) is a group of idiopathic chronic inflammatory intestinal conditions. To reveal its pathogenesis, several different animal models have been established. Although no single model captures the complexity of human IBD, each model provides valuable insights into one or another major aspect of disease, and together they have led to the establishment of a generally accepted understanding for human IBD pathogenesis. Recently, zebrafish have become an important platform for IBD research, and several murine IBD outcomes have been adapted to the zebrafish. Dextran Sulfate Sodium (DSS) -induced colitis, the most widely used murine IBD model, has never been successfully implemented in adult zebrafish. In this study we induced IBD in adult zebrafish by DSS with different methods, then compared its histological features and cytokine induction profiles with previous IBD models for validation. After successfully observing characteristics similar to murine IBD in the DSS-treated adult zebrafish, we reported the establishment of DSS-induced IBD adult zebrafish model.

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