Advisory Committee Chair
Louise Chow
Advisory Committee Members
Thomas Broker
David Crawford
Igor Chesnokov
Fang-Tsyr Lin
Weei-Chin Lin
Document Type
Dissertation
Date of Award
2008
Degree Name by School
Doctor of Philosophy (PhD) Heersink School of Medicine
Abstract
Papillomaviruses (PV) are prevalent pathogens that infect human or animal squamous epithelia. Its genome is a double strand circular DNA of approximately 7.9 kb. It contains origin of replication (ori) and encodes early viral proteins for viral DNA amplication, and late capsid proteins for packaging viron. The viral DNA replicates as extrachromosomal nuclear plasmid in the host cell. Infections by low risk virus, such as HPV-6 and -11, can result in benign papillomas, condylomata, and low grade squamous intraepithelial dysplasias (SIL), whereas infection by high risk virus, such as HPV-16 and -18, can progress to high grade SIL, carcinoma in situ, and cancers in which the viral genome may integrate into the host chromosome. Over 99% of cervical cancers are associated with high risk HPV infection. Currently, there is no reliable therapeutic treatment to HPV infections. Thus, it is important to have a thorough understanding on the regulation of viral DNA replication. HPV DNA replication required host DNA replication machinery and two of the viral proteins, the E2 ori binding protein and E1 replicative DNA helicase. E1 is a 68 kDa nuclear phosphoprotein and due to its high molecular weight, it can not enter nucleus by passive diffusion. In this dissertation, we investigated the mechanisms and regulation of E1 nuclear import, and the potential clinical application of our finding is discussed.
Recommended Citation
Yu, Jei-Hwa, "Mapks Regulate Nuclear Import Of Human Papillomavirus Type 11 Replicative Helicase E1" (2008). All ETDs from UAB. 3833.
https://digitalcommons.library.uab.edu/etd-collection/3833