Advisor(s)
Bryan Becker
Committee Member(s)
Chao He
Karen Gamble
School
Joint Health Sciences (Interdisciplinary)
Document Type
Thesis
Department (new version)
Neurobiology
Date of Award
9-11-2025
Abstract
Hypertension is a prevalent disease around the world. Increased blood pressure leads to a higher risk of stroke and coronary heart disease. Diet, lifestyle, and gene regulation are potential causative factors, and salt-sensitive hypertension is one common types of hypertension. In response to a high-salt diet, blood pressure may elevate with increasing risks of cardiovascular disease (CVD). Endothelin-1, which binds to endothelin A (ETA) and B (ETB) receptors, is strongly involved in controlling the blood vessels and sensory nerve signaling pathway between the kidney and the central nervous system during high salt dietary challenge. The function of the ETB receptor is important in regulating the decrease in blood pressure under different diets. Activation of ETB receptors usually results in blood vessel dilation. However, the role of ETB receptors on renal afferent nerves is less well known. We used rats deficient in ETB receptors under salty and normal salt diets. We found that there are differences in TRPV1 channel expressions between the high salt diet and the normal salt diet in vitro. The dorsal root ganglia cells collected from ETB-def rats on high salt diet had different activity of releasing Substance P. There is not much genomic research about dorsal root ganglia cells regarding salt-sensitive hypertension. But we used RNA bulk sequencing to evaluate the pathway of differentially expressed genes for both ETB-def rats and control rats under two different diets. Genetic analysis and cell culture research of this thesis provided insight for further research. We can better understand several causes of hypertension regarding of renal afferent nerve.
ProQuest ID
Recommended Citation
Shen, Jiaxi, "A High-Salt Diet Modulates Renal Afferent Nerve Activity; Potential Role Of The Endothelin System" (2025). All ETDs from UAB. 7349.
https://digitalcommons.library.uab.edu/etd-collection/7349
