Advisor(s)
Rakesh Kapoor
Committee Member(s)
Upender Mann
James C Martin
Document Type
Thesis
Date of Award
2008
Abstract
We report highly specific and sensitive detection of proteins in serum samples using a combination tapered fiber-optic biosensor (CTFOB) dip-probe. Sandwich immunoassay was used to generate specific fluorescence signal. A novel competitive adsorption strategy was used to reduce the false signal from non specific binding to a negligible level. Moreover, the similar strategy was also used to reduce the loss of target protein and increase the accuracy of immunoassay. The probes do not require pre-treatment with any blocking buffer. The specificity of the sensor was established by using negative control probes. The CTFOB probes were able to detect human interleukin-6 (IL-6) and interleukin-8 (IL-8) in the presence of much higher concentration (1 mg/ml) of a non-specific protein, specifically, IL-6 was detected down to a concentration of 5 pM (0.12 ng/ml) while IL-8 was detected down to 500 pM (4.2 ng/ml). These findings suggests that CTFOB probes can be used to detect analytes in different biologic fluids with high specificity and sensitivity; thus, can be used as a low-cost rapid tool in clinical diagnostic laboratories.
Recommended Citation
Wang, Chun-Wei, "Quantitative Protein Detection In Serum Samples Using Fiber-Optic Biosensors" (2008). All ETDs from UAB. 3648.
https://digitalcommons.library.uab.edu/etd-collection/3648
