Advisory Committee Chair
Rakesh Kapoor
Advisory Committee Members
Upender Mann
James C Martin
Document Type
Thesis
Date of Award
2008
Degree Name by School
Master of Science (MS) College of Arts and Sciences
Abstract
We report highly specific and sensitive detection of proteins in serum samples using a combination tapered fiber-optic biosensor (CTFOB) dip-probe. Sandwich immunoassay was used to generate specific fluorescence signal. A novel competitive adsorption strategy was used to reduce the false signal from non specific binding to a negligible level. Moreover, the similar strategy was also used to reduce the loss of target protein and increase the accuracy of immunoassay. The probes do not require pre-treatment with any blocking buffer. The specificity of the sensor was established by using negative control probes. The CTFOB probes were able to detect human interleukin-6 (IL-6) and interleukin-8 (IL-8) in the presence of much higher concentration (1 mg/ml) of a non-specific protein, specifically, IL-6 was detected down to a concentration of 5 pM (0.12 ng/ml) while IL-8 was detected down to 500 pM (4.2 ng/ml). These findings suggests that CTFOB probes can be used to detect analytes in different biologic fluids with high specificity and sensitivity; thus, can be used as a low-cost rapid tool in clinical diagnostic laboratories.
Recommended Citation
Wang, Chun-Wei, "Quantitative Protein Detection In Serum Samples Using Fiber-Optic Biosensors" (2008). All ETDs from UAB. 3648.
https://digitalcommons.library.uab.edu/etd-collection/3648